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Journal of Nanoparticle Research

Erschienen in:

01.09.2013 | Research Paper

Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells

verfasst von: Kumud Kant Awasthi, Anjali Awasthi, Narender Kumar, Partha Roy, Kamlendra Awasthi, P. J. John

Erschienen in: Journal of Nanoparticle Research | Ausgabe 9/2013

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Abstract

Silver nanoparticles (Ag NPs) are being used increasingly in wound dressings, catheters, and in various household products due to their antimicrobial activity. The present study reports the toxicity evaluation of synthesized and well characterized Ag NPs using Chinese hamster ovary (CHO) cells. The UV–Vis spectroscopy reveals the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 408–410 nm. Transmission electron microscopy (TEM) reveals that the average diameter of silver nanoparticles is about 5.0 ± 1.0 nm and that they have spherical shape. Cell visibility and cell viability percentage show dose-dependent cellular toxicity of Ag NPs. The half maximal inhibitory concentration (IC₅₀) for CHO cells is 68.0 ± 2.65 μg/ml after 24 h Ag NPs exposure. Toxicity evaluations, including cellular morphology, mitochondrial function (MTT assay), reactive oxygen species (ROS), and DNA fragmentation assay (Ladder pattern) were assessed in unexposed CHO cells (control) and the cells exposed to Ag NPs concentrations of 15, 30, and 60 μg/ml for 24 h. The findings may assist in the designing of Ag NPs for various applications and provide insights into their toxicity.

Vorheriger Artikel Poly(amido)amine (PAMAM) dendrimer–cisplatin complexes for chemotherapy of cisplatin-resistant ovarian cancer cells

Nächster Artikel Assessing interactions of hydrophilic nanoscale TiO2 with soil water

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Titel: Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells
verfasst von: Kumud Kant Awasthi
Anjali Awasthi
Narender Kumar
Partha Roy
Kamlendra Awasthi
P. J. John
Publikationsdatum: 01.09.2013
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 9/2013
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1898-5

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