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Erschienen in:

2014 | OriginalPaper | Buchkapitel

68. The Mechanism of Apoptosis in Adenocarcinoma of Lung Cancer A549 Cells Induced by Albumin-Derived from Peanut

verfasst von : Minhui Long, Yuejun Sun, Ni Chen, Zimin Zhang, Wen Chu, Yue Ma, Shenheng Luo, Zhongpeng Zhao, Aipo Diao

Erschienen in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Verlag: Springer Berlin Heidelberg

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Abstract

Peanut albumin which possesses protease inhibitor activity could be used as an anticancer regent. This paper would discuss the effect and mechanism of apoptosis induced by peanut albumin on human adenocarcinoma lung cancer A549. MTT assay was used to evaluate the inhibition of peanut albumin on 11 kinds of cancer cells. Compared with other cells, there was the great significant change in A549 cells. Morphological structures were detected by fluorescence microscope, and cell cycle was measured by flow cytometry (FCM). Annexin V/PI was used to detect cell cycle of A549 treated with peanut albumin. The differential expressions between the group treated with peanut albumin and the control were detected by Western-blot and quantitative-PCR. It was found that peanut albumin could inhibit proliferation of A549 cells, and induce apoptosis of A549. The possibility apoptosis of A549 cells induced by peanut albumin maybe correlated with mitochondrial apoptosis pathway.

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Titel: The Mechanism of Apoptosis in Adenocarcinoma of Lung Cancer A549 Cells Induced by Albumin-Derived from Peanut
verfasst von: Minhui Long
Yuejun Sun
Ni Chen
Zimin Zhang
Wen Chu
Yue Ma
Shenheng Luo
Zhongpeng Zhao
Aipo Diao
Verlag: Springer Berlin Heidelberg
Buch: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)
Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright-Jahr: 2014
DOI: https://doi.org/10.1007/978-3-642-37922-2_68

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