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Journal of Nanoparticle Research

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01.03.2017 | Research Paper

Constructing of DNA vectors with controlled nanosize and single dispersion by block copolymer coating gold nanoparticles as template assembly

verfasst von: Junbo Li, Wenlan Wu, Jiayu Gao, Ju Liang, Huiyun Zhou, Lijuan Liang

Erschienen in: Journal of Nanoparticle Research | Ausgabe 3/2017

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Abstract

Synthesized vectors with nanoscale size and stable colloid dispersion are highly desirable for improving gene delivery efficiency. Here, a core-shell template particle was constructed with polyethylene glycol-b-poly1-(3-aminopropyl)-3-(2-methacryloyloxy propylimidazolium bromine) (PEG-b-PAMPImB) coating gold nanoparticles (PEG-b-PAMPImB-@-Au NPs) for loading DNA and delivering in vitro. Data from transmission electron microscopy (TEM) and dynamic light scattering (DLS) suggest that these nanoplexes, by forming an electrostatic complex with DNA at the inner PAMPImB shell, offer steric protection for the outer PEG corona leading to single dispersion and small size. Notably, higher colloid stability and lower cytotoxicity were achieved with these nanoplexes when compared with PAMPImB monolayer-coated gold nanoparticles (Au NPs). Confocal laser scanning microscopy and intracellular trafficking TEM further indicate that the nanoplexes can translocate across the cell membrane and partly enter the nucleus for high efficient expression. Thus, template assembly represents a promising approach to control the size and colloid stability of gene vectors and ensure safety and efficiency of DNA delivery.

Vorheriger Artikel Effect of temperature on the synthesis of silver nanoparticles with polyethylene glycol: new insights into the reduction mechanism

Nächster Artikel Silver nanosheet-coated copper nanowire/epoxy resin nanocomposites with enhanced electrical conductivity and wear resistance

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Titel: Constructing of DNA vectors with controlled nanosize and single dispersion by block copolymer coating gold nanoparticles as template assembly
verfasst von: Junbo Li
Wenlan Wu
Jiayu Gao
Ju Liang
Huiyun Zhou
Lijuan Liang
Publikationsdatum: 01.03.2017
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 3/2017
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-017-3783-0

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