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Erschienen in: Colloid and Polymer Science 12/2011

01.08.2011 | Original Contribution

Effects of cationic and anionic nanoparticles on the stability of the secondary structure of DNA

verfasst von: Hiroyuki Kamata, Anatoly Zinchenko, Shizuaki Murata

Erschienen in: Colloid and Polymer Science | Ausgabe 12/2011

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Abstract

The effects of anionic and cationic nanoparticles (15 and 100 nm in diameter) on the stability of the secondary structure of DNA were studied in DNA denaturation and renaturation processes. The results showed that DNA denaturation is greatly inhibited in the presence of cationic nanoparticles. In contrast, in the presence of even large amounts of anionic nanoparticles, the DNA melting transition occurs at the original melting temperature. The effects of the size and concentration of nanoparticles on DNA denaturation are discussed.

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Literatur
1.
Zurück zum Zitat Sinden RR (1994) DNA Structure and Function. Academic, San Diego Sinden RR (1994) DNA Structure and Function. Academic, San Diego
2.
Zurück zum Zitat Bloomfield VA (1996) DNA condensation. Curr Opin Struct Biol 6(3):334–341CrossRef Bloomfield VA (1996) DNA condensation. Curr Opin Struct Biol 6(3):334–341CrossRef
3.
Zurück zum Zitat Uhlenhopp EL, Krasna AI (1969) Denaturation of DNA at pH 7.0 by acid and alkali. Nature 223(5212):1267–1269CrossRef Uhlenhopp EL, Krasna AI (1969) Denaturation of DNA at pH 7.0 by acid and alkali. Nature 223(5212):1267–1269CrossRef
4.
Zurück zum Zitat Tajmir-Riahi HA, Naoui M, Ahmad R (1993) The effects of Cu2+ and Pb2+ on the solution structure of calf thymus DNA: DNA condensation and denaturation studied by Fourier transform IR difference spectroscopy. Biopolymers 33(12):1819–1827CrossRef Tajmir-Riahi HA, Naoui M, Ahmad R (1993) The effects of Cu2+ and Pb2+ on the solution structure of calf thymus DNA: DNA condensation and denaturation studied by Fourier transform IR difference spectroscopy. Biopolymers 33(12):1819–1827CrossRef
5.
Zurück zum Zitat Morgan JE, Blankenship JW, Matthews HR (1986) Association constants for the interaction of double-stranded and single-stranded DNA with spermine, spermidine, putrescine, diaminopropane, N1- and N8-acetylspermidine, and magnesium: determination from analysis of the broadening of thermal denaturation curves. Arch Biochem Biophys 246(1):225–232CrossRef Morgan JE, Blankenship JW, Matthews HR (1986) Association constants for the interaction of double-stranded and single-stranded DNA with spermine, spermidine, putrescine, diaminopropane, N1- and N8-acetylspermidine, and magnesium: determination from analysis of the broadening of thermal denaturation curves. Arch Biochem Biophys 246(1):225–232CrossRef
6.
Zurück zum Zitat Kelly JM, Tossi AB, Mcconnell DJ, Ohuigin C (1985) A study of the interactions of some polypyridylruthenium(II) complexes with DNA using fluorescence spectroscopy, topoisomerization and thermal-denaturation. Nucleic Acids Research 13(17):6017–6034CrossRef Kelly JM, Tossi AB, Mcconnell DJ, Ohuigin C (1985) A study of the interactions of some polypyridylruthenium(II) complexes with DNA using fluorescence spectroscopy, topoisomerization and thermal-denaturation. Nucleic Acids Research 13(17):6017–6034CrossRef
7.
Zurück zum Zitat Aktipis S, Martz WW, Kindelis A (1975) Thermal denaturation of the DNA–ethidium complex. Redistribution of the intercalated dye during melting. Biochemistry 14(2):326–331CrossRef Aktipis S, Martz WW, Kindelis A (1975) Thermal denaturation of the DNA–ethidium complex. Redistribution of the intercalated dye during melting. Biochemistry 14(2):326–331CrossRef
9.
Zurück zum Zitat Thomas TJ, Kulkarni GD, Greenfield NJ, Shirahata A, Thomas T (1996) Structural specificity effects of trivalent polyamine analogues on the stabilization and conformational plasticity of triplex DNA. Biochem J 319(Pt 2):591–599 Thomas TJ, Kulkarni GD, Greenfield NJ, Shirahata A, Thomas T (1996) Structural specificity effects of trivalent polyamine analogues on the stabilization and conformational plasticity of triplex DNA. Biochem J 319(Pt 2):591–599
10.
Zurück zum Zitat Antony T, Thomas T, Shirahata A, Thomas TJ (1999) Selectivity of polyamines on the stability of RNA–DNA hybrids containing phosphodiester and phosphorothioate oligodeoxyribonucleotides. Biochemistry 38(33):10775–10784CrossRef Antony T, Thomas T, Shirahata A, Thomas TJ (1999) Selectivity of polyamines on the stability of RNA–DNA hybrids containing phosphodiester and phosphorothioate oligodeoxyribonucleotides. Biochemistry 38(33):10775–10784CrossRef
11.
Zurück zum Zitat Schindler T, Nordmeier E (1999) Denaturation experiments on calf-thymus DNA/polycation complexes in aqueous/organic solvent mixtures. Polymer 40(25):7019–7027CrossRef Schindler T, Nordmeier E (1999) Denaturation experiments on calf-thymus DNA/polycation complexes in aqueous/organic solvent mixtures. Polymer 40(25):7019–7027CrossRef
12.
Zurück zum Zitat Chen N, Zinchenko AA, Murata S, Yoshikawa K (2005) Specific formation of beads-on-a-chain structures on giant DNA using a designed polyamine derivative. J Am Chem Soc 127(31):10910–10916. doi:10.1021/ja042509q CrossRef Chen N, Zinchenko AA, Murata S, Yoshikawa K (2005) Specific formation of beads-on-a-chain structures on giant DNA using a designed polyamine derivative. J Am Chem Soc 127(31):10910–10916. doi:10.​1021/​ja042509q CrossRef
15.
Zurück zum Zitat Ganguli M, Babu JV, Maiti S (2004) Complex formation between cationically modified gold nanoparticles and DNA: an atomic force microscopic study. Langmuir 20(13):5165–5170. doi:10.1021/La036049a CrossRef Ganguli M, Babu JV, Maiti S (2004) Complex formation between cationically modified gold nanoparticles and DNA: an atomic force microscopic study. Langmuir 20(13):5165–5170. doi:10.​1021/​La036049a CrossRef
16.
Zurück zum Zitat Gvili K, Benny O, Danino D, Machluf M (2007) Poly(d, l-lactide-co-glycolide acid) nanoparticles for DNA delivery: waiving preparation complexity and increasing efficiency. Biopolymers 85(5–6):379–391. doi:10.1002/Bip.20697 Gvili K, Benny O, Danino D, Machluf M (2007) Poly(d, l-lactide-co-glycolide acid) nanoparticles for DNA delivery: waiving preparation complexity and increasing efficiency. Biopolymers 85(5–6):379–391. doi:10.​1002/​Bip.​20697
17.
Zurück zum Zitat Nafee N, Taetz S, Schneider M, Schaefer UF, Lehr CM (2007) Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides. Nanomed-Nanotechnol 3(3):173–183. doi:10.1016/j.nano.2007.03.006 CrossRef Nafee N, Taetz S, Schneider M, Schaefer UF, Lehr CM (2007) Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides. Nanomed-Nanotechnol 3(3):173–183. doi:10.​1016/​j.​nano.​2007.​03.​006 CrossRef
18.
Zurück zum Zitat Gemeinhart RA, Luo D, Saltzman WM (2005) Cellular fate of a modular DNA delivery system mediated by silica nanoparticles. Biotechnol Prog 21(2):532–537. doi:10.1021/Bp049648w CrossRef Gemeinhart RA, Luo D, Saltzman WM (2005) Cellular fate of a modular DNA delivery system mediated by silica nanoparticles. Biotechnol Prog 21(2):532–537. doi:10.​1021/​Bp049648w CrossRef
19.
Zurück zum Zitat Roy I, Ohulchanskyy TY, Bharali DJ, Pudavar HE, Mistretta RA, Kaur N, Prasad PN (2005) Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery. Proceedings of the National Academy of Sciences of the United States of America 102(2):279–284. doi:10.1073/pnas.0408039101 CrossRef Roy I, Ohulchanskyy TY, Bharali DJ, Pudavar HE, Mistretta RA, Kaur N, Prasad PN (2005) Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery. Proceedings of the National Academy of Sciences of the United States of America 102(2):279–284. doi:10.​1073/​pnas.​0408039101 CrossRef
21.
Zurück zum Zitat Kneuer C, Sameti M, Bakowsky U, Schiestel T, Schirra H, Schmidt H, Lehr CM (2000) A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro. Bioconjugate Chemistry 11(6):926–932. doi:10.1021/Bc0000637 CrossRef Kneuer C, Sameti M, Bakowsky U, Schiestel T, Schirra H, Schmidt H, Lehr CM (2000) A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro. Bioconjugate Chemistry 11(6):926–932. doi:10.​1021/​Bc0000637 CrossRef
23.
Zurück zum Zitat Xia TA, Kovochich M, Liong M, Meng H, Kabehie S, George S, Zink JI, Nel AE (2009) Polyethyleneimine coating enhances the cellular uptake of mesoporous silica nanoparticles and allows safe delivery of siRNA and DNA constructs. Acs Nano 3(10):3273–3286. doi:10.1021/Nn900918w CrossRef Xia TA, Kovochich M, Liong M, Meng H, Kabehie S, George S, Zink JI, Nel AE (2009) Polyethyleneimine coating enhances the cellular uptake of mesoporous silica nanoparticles and allows safe delivery of siRNA and DNA constructs. Acs Nano 3(10):3273–3286. doi:10.​1021/​Nn900918w CrossRef
24.
Zurück zum Zitat Zinchenko AA, Yoshikawa K, Baigl D (2005) Compaction of single-chain DNA by histone-inspired nanoparticles. Phys Rev Lett 95(22):228101CrossRef Zinchenko AA, Yoshikawa K, Baigl D (2005) Compaction of single-chain DNA by histone-inspired nanoparticles. Phys Rev Lett 95(22):228101CrossRef
25.
Zurück zum Zitat Zinchenko AA, Sakaue T, Araki S, Yoshikawa K, Baigl D (2007) Single-chain compaction of long duplex DNA by cationic nanoparticles: modes of interaction and comparison with chromatin. Journal of Physical Chemistry B 111(11):3019–3031. doi:10.1021/jp067926z CrossRef Zinchenko AA, Sakaue T, Araki S, Yoshikawa K, Baigl D (2007) Single-chain compaction of long duplex DNA by cationic nanoparticles: modes of interaction and comparison with chromatin. Journal of Physical Chemistry B 111(11):3019–3031. doi:10.​1021/​jp067926z CrossRef
26.
Zurück zum Zitat Takenaka T, Zinchenko AA, Yoshikawa K, Murata S (2010) Roles of mono- and divalent cations in DNA compaction induced by histone-mimic nanoparticles. Bulletin of the Chemical Society of Japan 83(6):643–650CrossRef Takenaka T, Zinchenko AA, Yoshikawa K, Murata S (2010) Roles of mono- and divalent cations in DNA compaction induced by histone-mimic nanoparticles. Bulletin of the Chemical Society of Japan 83(6):643–650CrossRef
28.
29.
Zurück zum Zitat Tan WL, Qin WJ, Yung LYL (2007) Difference in "base pair to termini" affects the enzymatic digestion of nanoparticle-bonded DNA. Biomacromolecules 8(2):750–752. doi:10.1021/Bm060904y CrossRef Tan WL, Qin WJ, Yung LYL (2007) Difference in "base pair to termini" affects the enzymatic digestion of nanoparticle-bonded DNA. Biomacromolecules 8(2):750–752. doi:10.​1021/​Bm060904y CrossRef
30.
Zurück zum Zitat Green M, Howman E (2005) Semiconductor quantum dots and free radical induced DNA nicking. Chemical communications (Cambridge, England) (1):121–123 Green M, Howman E (2005) Semiconductor quantum dots and free radical induced DNA nicking. Chemical communications (Cambridge, England) (1):121–123
31.
Zurück zum Zitat Anas A, Akita H, Harashima H, Itoh T, Ishikawa M, Biju V (2008) Photosensitized breakage and damage of DNA by CdSe-ZnS quantum dots. The Journal of Physical Chemistry B 112(32):10005–10011CrossRef Anas A, Akita H, Harashima H, Itoh T, Ishikawa M, Biju V (2008) Photosensitized breakage and damage of DNA by CdSe-ZnS quantum dots. The Journal of Physical Chemistry B 112(32):10005–10011CrossRef
34.
Zurück zum Zitat Jeon JH, Sung W, Ree FH (2006) A semiflexible chain model of local denaturation in double-stranded DNA. Journal of Chemical Physics 124 (16):-. doi:10.1063/1.2192774 Jeon JH, Sung W, Ree FH (2006) A semiflexible chain model of local denaturation in double-stranded DNA. Journal of Chemical Physics 124 (16):-. doi:10.​1063/​1.​2192774
35.
Zurück zum Zitat Storhoff JJ, Mirkin CA (1999) Programmed materials synthesis with DNA. Chem Rev 99(7):1849–1862CrossRef Storhoff JJ, Mirkin CA (1999) Programmed materials synthesis with DNA. Chem Rev 99(7):1849–1862CrossRef
37.
Zurück zum Zitat The estimation is made based on dependence of ζ potential of NP on amino groups concentration reported previously [26]. NP100(+) and NP15(+) were compared with NP100L and NP15 in [26] respectively. The estimation error does not exceed 20%. The estimation is made based on dependence of ζ potential of NP on amino groups concentration reported previously [26]. NP100(+) and NP15(+) were compared with NP100L and NP15 in [26] respectively. The estimation error does not exceed 20%.
38.
Metadaten
Titel
Effects of cationic and anionic nanoparticles on the stability of the secondary structure of DNA
verfasst von
Hiroyuki Kamata
Anatoly Zinchenko
Shizuaki Murata
Publikationsdatum
01.08.2011
Verlag
Springer-Verlag
Erschienen in
Colloid and Polymer Science / Ausgabe 12/2011
Print ISSN: 0303-402X
Elektronische ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-011-2453-5

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