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09.06.2017 | Original Paper

Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts

verfasst von: Daniela M. Ridolfi, Ana Paula Lemes, Sheila de Oliveira, Giselle Z. Justo, Marcelly V. Palladino, Nelson Durán

Erschienen in: Cellulose | Ausgabe 8/2017

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Abstract

Chitosan/poly(ethylene oxide) (PEO) (5:1) nanofibers with cellulose nanocrystals (CNCs) were produced using an electrospinning technique. The addition of CNCs to the chitosan/PEO solutions allowed the production of uniform fibers (without beads) with a high proportion of chitosan. The fiber diameters were influenced by the concentration of CNCs in the chitosan/PEO solutions. The solutions containing 10% (w/w) of CNCs produced thinner fibers compared to solutions containing 5% (w/w) of CNCs. Thermogravimetric analysis indicated that the nanofibers were thermally stable, despite the CNCs having an effect on the PEO decomposition. Results from the cell assay in cultures of 3T3 fibroblasts indicated that the chitosan/PEO nanofibers (with 10% CNCs) promoted cell attachment with changes in the cytoskeletal organization. The results obtained in this work highlight the favorable effect of CNCs in electrospinning of chitosan/PEO. As expected, the influence of nanofibers on 3T3 fibroblasts F-actin and β-tubulin network revealed alterations in cytoskeleton, leading to changes in cell morphology and spreading.

Vorheriger Artikel Bacterial cellulose based flexible multifunctional nanocomposite sheets

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Titel: Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts
verfasst von: Daniela M. Ridolfi
Ana Paula Lemes
Sheila de Oliveira
Giselle Z. Justo
Marcelly V. Palladino
Nelson Durán
Publikationsdatum: 09.06.2017
Verlag: Springer Netherlands
Erschienen in: Cellulose / Ausgabe 8/2017
Print ISSN: 0969-0239
Elektronische ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-017-1362-2

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