High sensitive immunoassay for endocrine disrupting chemicals using antibody immobilized microcapillary bundle structure

We proposed and fabricated a new chemical reactor with a vertical fluid flow operation for the wide biochemical applications. For rapid and high-yield reaction between reagents with large quantity, the filters, which possess 2,100 cylindrical through-bores were fabricated by deep X-ray lithography using synchrotron radiation. First, we tried the application of the fabricated vertical reactor to a sandwich enzyme-linked immunosorbent assay for the analysis of mouse immunoglobulin and nonylphenol using ultra violet absorption spectroscopy. We also confirmed the possibility of the analysis methods for competitive enzyme-linked immunosorbent assay of an endocrine disrupter, nonylphenol in a series of vertical fluidic operation. This sensitivity was one order higher than the sensitivity obtained by ordinary enzyme-linked immunosorbent assay using 96-wells microtiter plate and the same anti-NP-antibody.