Modern Techniques and Solvents for the Extraction of Microbial Oils

verfasst von: Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat

Verlag: Springer International Publishing

Buchreihe : SpringerBriefs in Molecular Science

Enthalten in: Springer Professional "Wirtschaft+Technik" , Springer Professional "Technik"

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Über dieses Buch

A valuable reference presenting many processes that facilitate lipid extraction from micro-organisms. Amongst the techniques included are Folch, Bligh and Dyer methods, and the Soxhlet technique as well as intensified green processes (ultrasound, microwave, supercritical fluid extraction, agro-solvent, accelerated solvent extraction, enzyme-assisted extraction, instant controlled pressure drop, pulse electric field). In addition to a section featuring the analysis of fatty acids by Gas Chromatography and lipids by High-Performance Thin-Layer Chromatography (HPTLC), this brief contains a valuable bibliography on microorganisms (classes, structures) and their applications as a source of value added oils and compounds for food and non-food applications such as biojet fuel.

Inhaltsverzeichnis

Frontmatter

Chapter 1. Microorganisms: Source of High Value Added Compounds

Abstract

This chapter presents the potential of primary and secondary metabolites produced by microorganisms after a brief introduction of their classification and detailing their cell wall structure. Furthermore, applications of microorganisms as sources of reagents and ingredients in various industries: food, cosmetic, pharmaceutics, nutraceutics, and biofuel.

Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat

Chapter 2. Analytical Methodology for Lipid Extraction and Quantification from Oleaginous Microorganisms

Abstract

This chapter reviews the development of extraction and analytical techniques for lipids from microorganisms. The commonly used extraction techniques of total lipid such as «Folch», «Bligh and Dyer», and Soxhlet Extraction methods are detailed and explained. A special focus has been made for the complementary analytical methods such as HP-TLC (High Performance Thin Layer Chromatography) which gives details about the repartition of the different classes of lipids and GC (Gas Chromatography) which brings quantitative analysis of all fatty acids chains present in the lipid microbial extract.

Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat

Chapter 3. Innovative Techniques and Alternative Solvents for Extraction of Microbial Oils

Abstract

This chapter reviews the recent development of extraction techniques, procedures and solvents for lipids from microorganisms. The modern innovative and intensified extraction techniques, alternative solvents and original procedures (ultrasound, microwave, supercritical fluid extraction, biobased-solvent, mechanical extraction, enzyme-assisted extraction, Instant controlled pressure drop, pulse electric field) are summarized in terms of their principles, processes, applications, benefits and disadvantages.

Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat

Chapter 4. Conclusion

Abstract

The use of innovative extraction techniques such as ultrasound, microwave, instant controlled pressure drop, supercritical fluid, pulsed electric fields, mechanical pressing, agro-solvent and enzyme assisted extraction (Table 4.1) allows total recovery of lipids from microorganisms and also reduced extraction time, energy consumed, and less solvent. Conventional techniques are limited by the diffusion of solvent into biomass, due to rigid structure of cell walls of microorganisms.

Alice Meullemiestre, Cassandra Breil, Maryline Abert-Vian, Farid Chemat

Backmatter

Titel: Modern Techniques and Solvents for the Extraction of Microbial Oils
verfasst von: Alice Meullemiestre
Cassandra Breil
Maryline Abert-Vian
Farid Chemat
Verlag: Springer International Publishing
Electronic ISBN: 978-3-319-22717-7
Print ISBN: 978-3-319-22716-0
DOI: https://doi.org/10.1007/978-3-319-22717-7