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2019 | OriginalPaper | Buchkapitel

9. Mechanical Load Transfer at the Cellular Level

verfasst von : Sara Barreto, Damien Lacroix

Erschienen in: Multiscale Mechanobiology in Tissue Engineering

Verlag: Springer Singapore

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Abstract

Cells interact with their extracellular environment, from which they gather information that influences their behaviour. The cytoskeleton provides a bridge to transmit information between the extracellular and the intracellular environments. It has been suggested that the CSK components may have distinct mechanical roles in the cell and that they might form the structure that defines cell rigidity. One approach to studying the mechanosensing processes is to understand the mechanical properties of cells’ constitutive components individually. In this chapter we describe the development of a multi-structural 3D finite element model of a single-adherent cell to investigate the biophysical differences of the mechanical role of each cytoskeleton component. The model includes prestressed actin bundles and microtubule within the cytoplasm and nucleus, which are surrounded by the actin cortex.

With the multi-structural model, we predicted that actin cortex and microtubules were targeted to respond to compressive loads, while actin bundles and microtubules were major components in maintaining cell forces during stretching. Additionally, corroboration of the multi-structural model regarding its ability to identify the role of the CSK components was obtained by comparing the numerical predictions with AFM force measurements on U2OS-osteosarcoma cells exposed to different cytoskeleton-disrupting drugs. Overall, the multi-structural model not only illustrates that a combination of cytoskeletal structures with their own properties is necessary for a complete description of cellular mechanics but also clarifies the effects of cytoskeletal heterogeneity on the interpretation of force-deformation measurements.

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Vorheriges Kapitel Computational Modelling of Collagen Hydrogel

Nächstes Kapitel Quantification of CSK Mechanics and Deformation in Relation to Cellular Functioning

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Titel: Mechanical Load Transfer at the Cellular Level
verfasst von: Sara Barreto
Damien Lacroix
Verlag: Springer Singapore
Buch: Multiscale Mechanobiology in Tissue Engineering
Print ISBN: 978-981-10-8074-6

Electronic ISBN: 978-981-10-8075-3

Copyright-Jahr: 2019
DOI: https://doi.org/10.1007/978-981-10-8075-3_9

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