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Journal of Materials Science
Erschienen in: Journal of Materials Science 20/2014

01.10.2014 | Original Paper

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

verfasst von: Yakun Hou, Pingxiao Wu, Zhujian Huang, Bo Ruan, Paiyu Liu, Nengwu Zhu

Erschienen in: Journal of Materials Science | Ausgabe 20/2014

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Abstract

Montmorillonite (MMT) and rectorite (REC) are clay minerals that consist of hydrated aluminum silicate with Si–O tetrahedrons on the bottom of the layer, Al–O(OH)2 octahedrons on the top, and various exchangeable ions such as Na+ and Ca2+ in interlayer. However, the driving force for intercalation of double-stranded DNA is not sufficient to open up the gallery of MMT and REC and allows the intercalation to occur. Furthermore, the external surface of MMT and REC is hydrophilic rather than organophilic. In the study, cationic hexadecyl trimethyl ammonium bromide (CTAB) is chosen to modify MMT and REC to make clay minerals more compatible with DNA. CTAB intercalates into the galleries of clay minerals and expands the basal spacing for DNA intercalation. Novel CTAB-clay/DNA hybrids are synthesized for the first time, with their structure investigated by X-ray diffraction and Fourier transform infrared. Gel electrophoresis analysis confirms that the CTAB-modified clay could protect DNA from degradation of DNase I. UV absorption spectroscopy and circular dichroism indicate that the modified clay minerals can provide a capacity for protecting DNA from damage induced by heavy metals. In addition, the intercalated DNA can be recovered readily under alkaline conditions. Therefore, CTAB-clay/DNA hybrids are potential materials for storage of genetic information.
Vorheriger Artikel Interatomic potential to predict the glass-forming ability of Ni–Nb–Mo ternary alloys
Nächster Artikel Phase transformation behavior of zinc metastannates obtained by aqueous precipitation at different temperatures

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Metadaten
Titel
Successful intercalation of DNA into CTAB-modified clay minerals for gene protection
verfasst von
Yakun Hou
Pingxiao Wu
Zhujian Huang
Bo Ruan
Paiyu Liu
Nengwu Zhu
Publikationsdatum
01.10.2014
Verlag
Springer US
Erschienen in
Journal of Materials Science / Ausgabe 20/2014
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-014-8435-5

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