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13.09.2022 | Original Article

The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni²⁺ ion removal from the contaminated water

verfasst von: Verica Ljubic, Milena Milosevic, Slobodan Cvetkovic, Marijana Stojanovic, Katarina Novovic, Miroslav Dinic, Mina Popovic

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 10/2024

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Abstract

As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni²⁺ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni²⁺ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.

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Vorheriger Artikel Synthesis and characterization of bio-based UV curable polyurethane coatings from algal biomass residue

Nächster Artikel Sustainable removal of methylene blue dye from textile effluent by using cellulose nanocrystals extracted from sugarcane bagasse

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Hooshdar P, Kermanshahi RK, Ghadam P, Khosravi-Darani K (2020) A review on production of exopolysaccharide and biofilm in probiotics like lactobacilli and methods of analysis. Biointerface Res Appl Chem 10:6058–6075. https://doi.org/10.33263/BRIAC105.60586075CrossRef

Mehta K, Shukla A, Saraf M (2021) Articulating the exuberant intricacies of bacterial exopolysaccharides to purge environmental pollutants. Heliyon 7:e08446. https://doi.org/10.1016/j.heliyon.2021.e08446CrossRef

Segers ME, Lebeer S (2014) Towards a better understanding of Lactobacillus rhamnosus GG - host interactions. Microb Cell Fact 13:1–16. https://doi.org/10.1186/1475-2859-13-S1-S7CrossRef

Freitas F, Alves VD, Reis MAM (2011) Advances in bacterial exopolysaccharides: from production to biotechnological applications. Trends Biotechnol 29:388–398. https://doi.org/10.1016/j.tibtech.2011.03.008CrossRef

Abid Y, Casillo A, Gharsallah H et al (2018) Production and structural characterization of exopolysaccharides from newly isolated probiotic lactic acid bacteria. Int J Biol Macromol 108:719–728. https://doi.org/10.1016/j.ijbiomac.2017.10.155CrossRef

Chen YC, Wu YJ, Hu CY (2019) Monosaccharide composition influence and immunomodulatory effects of probiotic exopolysaccharides. Int J Biol Macromol 133:575–582. https://doi.org/10.1016/j.ijbiomac.2019.04.109CrossRef

Ashok Kumar Nadda Sajna K. V. Swati Sharma (2021) Exopolysaccharides as novel and significant biomaterials, Springer Series on Polymer and Composite Materials (SSPCM), ISBN: 978–3–030–75289–7

Mohite BV, Koli SH, Narkhede CP et al (2017) Prospective of microbial exopolysaccharide for heavy metal exclusion. Appl Biochem Biotechnol 183:582–600. https://doi.org/10.1007/s12010-017-2591-4CrossRef

Ibrahim IM, Konnova SA, Sigida EN et al (2020) Bioremediation potential of a halophilic Halobacillus sp. strain, EG1HP4QL: exopolysaccharide production, crude oil degradation, and heavy metal tolerance. Extremophiles 24:157–166. https://doi.org/10.1007/s00792-019-01143-2CrossRef

10.

Fredrik Levander, Malin Svensson and PR (2002) Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus. Appl Environ Microbiol. https://doi.org/10.1128/AEM.68.11.5656-5662.2002

11.

Concórdio-Reis P, Reis MAM, Freitas F (2020) Biosorption of heavy metals by the bacterial exopolysaccharide fucopol. Appl Sci 10. https://doi.org/10.3390/APP10196708

12.

Zeraatkar AK, Ahmadzadeh H, Talebi AF et al (2016) Potential use of algae for heavy metal bioremediation, a critical review. J Environ Manage 181:817–831. https://doi.org/10.1016/j.jenvman.2016.06.059CrossRef

13.

Lu H, Shi X, Costa M, Huang C (2005) Carcinogenic effect of nickel compounds. Mol Cell Biochem 279:45–67. https://doi.org/10.1007/s11010-005-8215-2CrossRef

14.

Shrestha R, Ban S, Devkota S et al (2021) Technological trends in heavy metals removal from industrial wastewater: a review. J Environ Chem Eng. 9:105688. https://doi.org/10.1016/j.jece.2021.105688CrossRef

15.

Dobrowolski R, Szcześ A, Czemierska M, Jarosz-Wikołazka A (2017) Studies of cadmium(II), lead(II), nickel(II), cobalt(II) and chromium(VI) sorption on extracellular polymeric substances produced by Rhodococcus opacus and Rhodococcus rhodochrous. Bioresour Technol 225:113–120. https://doi.org/10.1016/j.biortech.2016.11.040CrossRef

16.

Li H, Dong W, Liu Y et al (2019) Enhanced biosorption of nickel ions on immobilized surface-engineered yeast using nickel-binding peptides. Front Microbiol 10. https://doi.org/10.3389/fmicb.2019.01254

17.

Popović M, Stojanović M, Veličković Z et al (2021) Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. Int J Biol Macromol 183:423–434. https://doi.org/10.1016/j.ijbiomac.2021.04.177CrossRef

18.

Bajpai VK, Majumder R, Rather IA, Kim K (2016) Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method. Bangladesh J Pharmacol 11:573–576. https://doi.org/10.3329/bjp.v11i3.27170CrossRef

19.

Sims IM, Frese SA, Walter J et al (2011) Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri. 100–23. ISME J 5:1115–1124. https://doi.org/10.1038/ismej.2010.201CrossRef

20.

Xu Y, Cui Y, Wang X et al (2019) Purification, characterization and bioactivity of exopolysaccharides produced by Lactobacillus plantarum KX041. Int J Biol Macromol 128:480–492. https://doi.org/10.1016/j.ijbiomac.2019.01.117CrossRef

21.

Tiwari ON, Mondal A, Bhunia B et al (2019) Purification, characterization and biotechnological potential of new exopolysaccharide polymers produced by cyanobacterium Anabaena sp. CCC 745. Polymer (Guildf). 178:121695. https://doi.org/10.1016/j.polymer.2019.121695

22.

Gonzalez-Gil G, Thomas L, Emwas AH et al (2015) NMR and MALDI-TOF MS based characterization of exopolysaccharides in anaerobic microbial aggregates from full-scale reactors. Sci Rep 5:1–12. https://doi.org/10.1038/srep14316CrossRef

23.

Mitra I, Mukherjee S, Reddy Venkata PB et al (2016) Benzimidazole based Pt(II) complexes with better normal cell viability than cisplatin: synthesis, substitution behavior, cytotoxicity, DNA binding and DFT study. RSC Adv 6:76600–76613. https://doi.org/10.1039/c6ra17788cCrossRef

24.

Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302. https://doi.org/10.1021/jf0502698CrossRef

25.

Milošević MD, Marinković AD, Petrović P et al (2020) Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. Bioorg Chem 102:104073. https://doi.org/10.1016/j.bioorg.2020.104073CrossRef

26.

Mustafa S, Dilara B, Nargis K et al (2002) Surface properties of the mixed oxides of iron and silica. Colloids Surfaces A Physicochem Eng Asps 205:273–282. https://doi.org/10.1016/S0927-7757(02)00025-0CrossRef

27.

Li H, Liu L, Cui J et al (2020) High-efficiency adsorption and regeneration of methylene blue and aniline onto activated carbon from waste edible fungus residue and its possible mechanism. RSC Adv 10:14262–14273. https://doi.org/10.1039/d0ra01245aCrossRef

28.

Wang X, Shao C, Liu L et al (2017) Optimization, partial characterization and antioxidant activity of an exopolysaccharide from Lactobacillus plantarum KX041. Int J Biol Macromol 103:1173–1184. https://doi.org/10.1016/j.ijbiomac.2017.05.118CrossRef

29.

Charoenwongpaiboon T, Wangpaiboon K, Pichyangkura R et al (2021) Characterization of a nanoparticulate exopolysaccharide from Leuconostoc holzapfelii KM01 and its potential application in drug encapsulation. Int J Biol Macromol 187:690–698. https://doi.org/10.1016/j.ijbiomac.2021.07.174CrossRef

30.

Wangpaiboon K, Waiyaseesang N, Panpetch P et al (2020) Characterisation of insoluble α-1,3-/α-1,6 mixed linkage glucan produced in addition to soluble α-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1. Int J Biol Macromol 152:473–482. https://doi.org/10.1016/j.ijbiomac.2020.02.247CrossRef

31.

Wang J, Ai H, Liu M (2014) Enhanced welan gum production using cane molasses as substrate by Alcaligenes sp ATCC31555. N Biotechnol 31:S40. https://doi.org/10.1016/j.nbt.2014.05.1704CrossRef

32.

Melo MRS, Feitosa JPA, Freitas ALP, De Paula RCM (2002) Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea. Carbohydr Polym 49:491–498. https://doi.org/10.1016/S0144-8617(02)00006-1CrossRef

33.

Wang J, Zhao X, Yang Y et al (2015) Characterization and bioactivities of an exopolysaccharide produced by Lactobacillus plantarum YW32. Int J Biol Macromol 74:119–126. https://doi.org/10.1016/j.ijbiomac.2014.12.006CrossRef

34.

Ermiş E, Poyraz E, Dertli E, Yılmaz MT (2020) Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization. Sak Univ J Sci. 151–160. https://doi.org/10.16984/saufenbilder.545929

35.

Wu J, Yan D, Liu Y, et al (2021) Purification, structural characteristics, and biological activities of exopolysaccharide isolated from Leuconostoc mesenteroides SN-8. Front Microbiol 12. https://doi.org/10.3389/fmicb.2021.644226

36.

Meng M, Cheng D, Han L et al (2017) Isolation, purification, structural analysis and immunostimulatory activity of water-soluble polysaccharides from Grifola frondosa fruiting body. Carbohydr Polym 157:1134–1143. https://doi.org/10.1016/j.carbpol.2016.10.082CrossRef

37.

Chi Z, Su CD, Lu WD (2007) A new exopolysaccharide produced by marine Cyanothece sp. 113. Bioresour Technol 98:1329–1332. https://doi.org/10.1016/j.biortech.2006.05.001CrossRef

38.

Shuhong Y, Meiping Z, Hong Y et al (2014) Biosorption of Cu2+, Pb2+ and Cr6+ by a novel exopolysaccharide from Arthrobacter ps-5. Carbohydr Polym 101:50–56. https://doi.org/10.1016/j.carbpol.2013.09.021CrossRef

39.

Braissant O, Decho AW, Przekop KM et al (2009) Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat. FEMS Microbiol Ecol 67:293–307. https://doi.org/10.1111/j.1574-6941.2008.00614.xCrossRef

40.

Kanamarlapudi SLRK, Muddada S (2017) Characterization of exopolysaccharide produced by Streptococcus thermophilus CC30. Biomed Res Int 2017. https://doi.org/10.1155/2017/4201809

41.

Rajoka MSR, Mehwish HM, Hayat HF et al (2019) Correction to: Characterization, the antioxidant and antimicrobial activity of exopolysaccharide isolated from poultry origin lactobacilli (probiotics and antimicrobial proteins, (2018). Probiotics Antimicrob Proteins 11:1143–1144. https://doi.org/10.1007/s12602-018-9504-xCrossRef

42.

Liu Z, Zhang Z, Qiu L et al (2017) Characterization and bioactivities of the exopolysaccharide from a probiotic strain of Lactobacillus plantarum WLPL04. J Dairy Sci 100:6895–6905. https://doi.org/10.3168/jds.2016-11944CrossRef

43.

Wang Y, Li C, Liu P et al (2010) Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir. Carbohydr Polym 82:895–903. https://doi.org/10.1016/j.carbpol.2010.06.013CrossRef

44.

Saravanan C, Shetty PKH (2016) Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter. Int J Biol Macromol 90:100–106. https://doi.org/10.1016/j.ijbiomac.2015.02.007CrossRef

45.

Mosharaf MK, Tanvir MZH, Haque MM et al (2018) Metal-adapted bacteria isolated from wastewaters produce biofilms by expressing proteinaceous curli fimbriae and cellulose nanofibers. Front Microbiol 9:1–17. https://doi.org/10.3389/fmicb.2018.01334CrossRef

46.

Krishnamurthy M, JayaramanUthaya C, Thangavel M et al (2020) Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3–1. Carbohydr Polym. 227:115369. https://doi.org/10.1016/j.carbpol.2019.115369CrossRef

47.

Chowdhury SR, Basak RK, Sen R, Adhikari B (2011) Characterization and emulsifying property of a carbohydrate polymer produced by Bacillus pumilus UW-02 isolated from waste water irrigated agricultural soil. Int J Biol Macromol. 48:705–712. https://doi.org/10.1016/j.ijbiomac.2011.02.019CrossRef

48.

Wang B, Song Q, Zhao F et al (2019) Purification and characterization of dextran produced by Leuconostoc pseudomesenteroides PC as a potential exopolysaccharide suitable for food applications. Process Biochem 87:187–195. https://doi.org/10.1016/j.procbio.2019.08.020CrossRef

49.

Miao M, Ma Y, Huang C et al (2015) Physicochemical properties of a water soluble extracellular homopolysaccharide from Lactobacillus reuteri SK24.003. Carbohydr PolymS 131:377–383. https://doi.org/10.1016/j.carbpol.2015.05.066CrossRef

50.

Sharma K, Sharma N, Handa S, Pathania S (2020) Purification and characterization of novel exopolysaccharides produced from Lactobacillus paraplantarum KM1 isolated from human milk and its cytotoxicity. J Genet Eng Biotechnol 18. https://doi.org/10.1186/s43141-020-00063-5

51.

Monteagudo-Mera A, Rastall RA, Gibson GR et al (2019) Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health. Appl Microbiol Biotechnol 103:6463–6472. https://doi.org/10.1007/s00253-019-09978-7CrossRef

52.

Li W, Ji J, Chen X et al (2014) Structural elucidation and antioxidant activities of exopolysaccharides from Lactobacillus helveticus MB2–1. Carbohydr Polym 102:351–359. https://doi.org/10.1016/j.carbpol.2013.11.053CrossRef

53.

Li L, Thakur K, Liao BY, et al (2018) Antioxidant and antimicrobial potential of polysaccharides sequentially extracted from Polygonatum cyrtonema Hua. Elsevier B.V

54.

Yu X, Zhang G, Xie C et al (2011) Equilibrium, kinetic, and thermodynamic studies of hazardous dye neutral red biosorption by spent corncob substrate. BioResources 6:936–949CrossRef

55.

Wei W, Wang Q, Li A, et al (2016) Biosorption of Pb (II) from aqueous solution by extracellular polymeric substances extracted from Klebsiella sp. J1: adsorption behavior and mechanism assessment. Sci Rep. 6:1–10. https://doi.org/10.1038/srep31575

56.

Öztürk A, Artan T, Ayar A (2004) Biosorption of nickel(II) and copper(II) ions from aqueous solution by Streptomyces coelicolor A3(2). Colloids Surfaces B Biointerfaces. 34:105–111. https://doi.org/10.1016/j.colsurfb.2003.11.008CrossRef

57.

Zhang Z, Cai R, Zhang W, et al (2017) A novel exopolysaccharide with metal adsorption capacity produced by a marine bacterium Alteromonas sp. JL2810. Mar Drugs 15. https://doi.org/10.3390/md15060175

58.

Petrović M, Šoštarić T, Stojanović M et al (2017) Mechanism of adsorption of Cu2+ and Zn2+ on the corn silk (Zea mays L.). Ecol Eng. 99:83–90. https://doi.org/10.1016/j.ecoleng.2016.11.057CrossRef

59.

Reddy DHK, Lee SM, Seshaiah K (2012) Biosorption of toxic heavy metal ions from water environment using honeycomb biomass-an industrial waste material. Water Air Soil Pollut 223:5967–5982. https://doi.org/10.1007/s11270-012-1332-0CrossRef

60.

Zafar MN, Aslam I, Nadeem R et al (2015) Characterization of chemically modified biosorbents from rice bran for biosorption of Ni(II). J Taiwan Inst Chem Eng 46:82–88. https://doi.org/10.1016/j.jtice.2014.08.034CrossRef

61.

Malkoc E, Nuhoglu Y (2007) Determination of kinetic and equilibrium parameters of the batch adsorption of Cr(VI) onto waste acorn of Quercus ithaburensis. Chem Eng Process Process Intensif 46:1020–1029. https://doi.org/10.1016/j.cep.2007.05.007CrossRef

62.

Gupta P, Diwan B (2017) Bacterial exopolysaccharide mediated heavy metal removal: a review on biosynthesis, mechanism and remediation strategies. Biotechnol Reports 13:58–71. https://doi.org/10.1016/j.btre.2016.12.006CrossRef

63.

Lakzian A, Berenji AR, Karimi E, Razavi S (2008) Adsorption capability of lead, nickel and zinc by exopolysaccharide and dried cell of Ensifer meliloti. Asian J Chem 20:6075–6080

64.

Cui D, Tan C, Deng H, et al (2020) Biosorption mechanism of aqueous Pb2+, Cd2+, and Ni2+ions on extracellular polymeric substances (EPS). Archaea 2020. https://doi.org/10.1155/2020/8891543

65.

Pagliaccia B, Carretti E, Severi M et al (2022) Heavy metal biosorption by extracellular polymeric substances (EPS) recovered from anammox granular sludge. J Hazard Mater. 424:126661. https://doi.org/10.1016/j.jhazmat.2021.126661CrossRef

66.

Chug R, Mathur S, Kothari SL et al (2021) Maximizing EPS production from Pseudomonas aeruginosa and its application in Cr and Ni sequestration. Biochem Biophys Reports 26:100972. https://doi.org/10.1016/j.bbrep.2021.100972CrossRef

Titel: The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water
verfasst von: Verica Ljubic
Milena Milosevic
Slobodan Cvetkovic
Marijana Stojanovic
Katarina Novovic
Miroslav Dinic
Mina Popovic
Publikationsdatum: 13.09.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Biomass Conversion and Biorefinery / Ausgabe 10/2024
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-022-03292-5

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