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Erschienen in: Natural Computing 2/2008

01.06.2008

Unravel four hairpins!

verfasst von: Atsushi Kameda, Masahito Yamamoto, Azuma Ohuchi, Satsuki Yaegashi, Masami Hagiya

Erschienen in: Natural Computing | Ausgabe 2/2008

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Abstract

DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.

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Metadaten
Titel
Unravel four hairpins!
verfasst von
Atsushi Kameda
Masahito Yamamoto
Azuma Ohuchi
Satsuki Yaegashi
Masami Hagiya
Publikationsdatum
01.06.2008
Verlag
Springer Netherlands
Erschienen in
Natural Computing / Ausgabe 2/2008
Print ISSN: 1567-7818
Elektronische ISSN: 1572-9796
DOI
https://doi.org/10.1007/s11047-007-9035-y

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