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Journal of Materials Science
Erschienen in: Journal of Materials Science 23/2014

01.12.2014 | Original Paper

Preparation of an enzyme/inorganic nanosheet/magnetic bead complex and its enzymatic activity

verfasst von: Nobuaki Soh, Shunsuke Kaneko, Kazuhiro Uozumi, Toshihisa Ueda, Kai Kamada

Erschienen in: Journal of Materials Science | Ausgabe 23/2014

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Abstract

A complex composed of enzyme (horseradish peroxidase, HRP), layered titanate (TiOx), and magnetic beads (MB) was prepared, and the enzymatic activity of the complex and the repeatable use for the enzyme in the complex were investigated. The HRP/TiOx/MB complex was formed in acetate buffer (pH 4) by electrostatic interaction among the components where HRP and MB have positive charge, whereas TiOx is negatively charged. The resultant complex was sufficiently stable in an aqueous solution, and HRP immobilized on the layered titanate in the complex kept a certain enzymatic activity in which typical enzymatic relationship was observed between substrate concentration and initial reaction rate. By giving a magnetic field using commercial magnets, the HRP/TiOx/MB complex was easily and rapidly recovered, and the recovered complex was able to be used for the next enzymatic reactions. The enzyme/inorganic nanosheet/MB complex proposed in this study is expected to be applied as a new tool in the bioengineering and nanobiotechnological fields.
Vorheriger Artikel Controlled hydrothermal growth of multi-length-scale ZnO nanowires using liquid masking layers
Nächster Artikel Preparation and performance evaluation of castor oil-based polyurethane prepolymer/polylactide blends

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Metadaten
Titel
Preparation of an enzyme/inorganic nanosheet/magnetic bead complex and its enzymatic activity
verfasst von
Nobuaki Soh
Shunsuke Kaneko
Kazuhiro Uozumi
Toshihisa Ueda
Kai Kamada
Publikationsdatum
01.12.2014
Verlag
Springer US
Erschienen in
Journal of Materials Science / Ausgabe 23/2014
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-014-8508-5

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