Skip to main content
Fachgebiete
Automobil + Motoren Bauwesen + Immobilien Business IT + Informatik Elektrotechnik + Elektronik Energie + Nachhaltigkeit Finance + Banking Management + Führung Marketing + Vertrieb Maschinenbau + Werkstoffe Versicherung + Risiko
DE
EN
Themenseiten
Organisationspsychologie Projektmanagement Marketing Smart Manufacturing
Bücher
Zeitschriften
Weitere Formate
Podcasts Webinare Technik Webinare Wirtschaft Kongresse Veranstaltungskalender Awards
Jetzt Einzelzugang starten
Zugang für Unternehmen
Referenzkunden
MTZ-Fachkongress

Antriebe und Energiesysteme von morgen


Austausch von Automobil- und Energiewirtschaft

Am 14./15. Mai geht es in Chemnitz um die Mobilitätswende durch Elektro- und Wasserstofffahrzeuge.
Erweiterte Suche
Anmelden
nach oben
Journal of Materials Science
Erschienen in: Journal of Materials Science 15/2018

04.05.2018 | Biomaterials

Development of biocompatible fluorescent gelatin nanocarriers for cell imaging and anticancer drug targeting

verfasst von: Parinaz Nezhad-Mokhtari, Nasser Arsalani, Marjan Ghorbani, Hamed Hamishehkar

Erschienen in: Journal of Materials Science | Ausgabe 15/2018

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config
loading …

Abstract

In recent years, fluorescent carbon dots (CDs) have attracted a great deal of attention in imaging and related biomedical applications due to their excellent photoluminescence properties, low cost, high quantum yield and low cytotoxicity in comparison with semiconductor quantum dots based on metallic elements. In this paper, a new and simple design for development of CDs/gelatin nanoparticles (CDs/GNPs) is described which used as a novel methotrexate (MTX) nanocarrier and MCF-7 cell imaging. The obtained fluorescent nanocarriers were characterized using FTIR, SEM, XRD, DLS, PL, TGA, and zeta-potential analysis. Afterward, the performance of developed NPs was investigated through different in vitro tests such as MTT assay, fluorescence microscopy, and flow cytometry analyses. MTX was successfully loaded into the fluorescent NPs at physiological pH (7.4) by ionic interactions between anionic carboxylate groups of MTX and cationic amino groups on the surface of NPs. MTX releasing ability of the obtained nanocarrier was illustrated through the comparison of in vitro drug release at both simulated tumor tissue and physiological environment. The MTT assay revealed that the MTX-loaded nanocarriers have higher cytotoxicity in MCF-7 breast cancer cells than nanocarriers without MTX. Upon the obtained results, our fluorescent nanocarriers hold great potential as drug delivery carriers for the targeted MTX delivery to the cancer cells and biological fluorescent labeling.
Vorheriger Artikel Structure, mechanical properties and surface morphology of the snapping shrimp claw
Nächster Artikel Microcrystals of antimony compounds in lead–potassium and lead glass and their effect on glass corrosion: a study of historical glass beads using electron microscopy

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Jetzt informieren
Literatur
1.
Smith BR, Gambhir SS (2017) Nanomaterials for in vivo imaging. Chem Rev 117(3):901–986CrossRef
2.
Kim S, Choi Y, Park G, Won C, Park Y-J, Lee Y, Kim B-S, Min D-H (2017) Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo. Nano Res 10(2):503–519CrossRef
3.
Gun’ko YK (2016) Nanoparticles in bioimaging. Multidisciplinary Digital Publishing Institute, Basel
4.
Jin G, He R, Liu Q, Dong Y, Lin M, Li W, Xu F (2018) Theranostics of triple-negative breast cancer based on conjugated polymer nanoparticles. ACS Appl Mater Interfaces 10(13):10634–10646CrossRef
5.
Jin G, Zhao X, Xu F (2017) Therapeutic nanomaterials for cancer therapy and tissue regeneration. Drug Discov Today 22(9):1285–1287CrossRef
6.
Jin G, Feng G, Qin W, Tang BZ, Liu B, Li K (2016) Multifunctional organic nanoparticles with aggregation-induced emission (AIE) characteristics for targeted photodynamic therapy and RNA interference therapy. Chem Commun 52(13):2752–2755CrossRef
7.
Zhu S, Song Y, Zhao X, Shao J, Zhang J, Yang B (2015) The photoluminescence mechanism in carbon dots (graphene quantum dots, carbon nanodots, and polymer dots): current state and future perspective. Nano Res 8(2):355–381CrossRef
8.
Sundar S, Kundu J, Kundu SC (2010) Biopolymeric nanoparticles. Sci Technol Adv Mater 11(1):014104CrossRef
9.
Kamaly N, Xiao Z, Valencia PM, Radovic-Moreno AF, Farokhzad OC (2012) Targeted polymeric therapeutic nanoparticles: design, development and clinical translation. Chem Soc Rev 41(7):2971–3010CrossRef
10.
Zeng Q, Shao D, He X, Ren Z, Ji W, Shan C, Qu S, Li J, Chen L, Li Q (2016) Carbon dots as a trackable drug delivery carrier for localized cancer therapy in vivo. J Mater Chem B 4(30):5119–5126CrossRef
11.
Kang Z, Liu Y, Lee S-T (2017) Carbon dots for bioimaging and biosensing applications. In: Springer series on chemical sensors and biosensors (methods and applications). Springer, Berlin, Heidelberg, pp 1–31
12.
Jelinek R (2017) Biological applications of carbon-dots. In: Carbon quantum dots. Springer, pp 47–60
13.
Foox M, Zilberman M (2015) Drug delivery from gelatin-based systems. Exp Opin Drug Deliv 12(9):1547–1563CrossRef
14.
Mironova M, Kovaleva E (2017) Comparative analysis of quality assessment requirements for gelatin used in drug production. Pharm Chem J 50(12):820–825CrossRef
15.
Liu S, Liu X, Han MY (2016) Controlled modulation of surface coating and surface charging on quantum dots with negatively charged gelatin for substantial enhancement and reversible switching in photoluminescence. Adv Funct Mater 26(48):8991–8998CrossRef
16.
Huang G, Li F, Zhao X, Ma Y, Li Y, Lin M, Jin G, Lu TJ, Genin GM, Xu F (2017) Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment. Chem Rev 117(20):12764–12850CrossRef
17.
MaHam A, Tang Z, Wu H, Wang J, Lin Y (2009) Protein-based nanomedicine platforms for drug delivery. Small 5(15):1706–1721CrossRef
18.
Kumari A, Yadav SK, Yadav SC (2010) Biodegradable polymeric nanoparticles based drug delivery systems. Colloids Surf B Biointerfaces 75(1):1–18CrossRef
19.
Byrne SJ, Williams Y, Davies A, Corr SA, Rakovich A, Gun’ko YK, Rakovich YP, Donegan JF, Volkov Y (2007) “Jelly dots”: synthesis and cytotoxicity studies of CdTe quantum dot–gelatin nanocomposites. Small 3(7):1152–1156CrossRef
20.
Chen L, Siemiarczuk A, Hai H, Chen Y, Huang G, Zhang J (2014) Development of biocompatible and proton-resistant quantum dots assembled on gelatin nanospheres. Langmuir 30(7):1893–1899CrossRef
21.
Wang Y, Chen H, Ye C, Hu Y (2008) Synthesis and characterization of CdTe quantum dots embedded gelatin nanoparticles via a two-step desolvation method. Mater Lett 62(19):3382–3384CrossRef
22.
Chen L, Willoughby A, Zhang J (2014) Luminescent gelatin nanospheres by encapsulating CdSe quantum dots. Luminescence 29(1):74–78CrossRef
23.
Girija Aswathy R, Sivakumar B, Brahatheeshwaran D, Ukai T, Yoshida Y, Maekawa T, Kumar SD (2011) Biocompatible fluorescent jelly quantum dots for bioimaging. Mater Express 1(4):291–298CrossRef
24.
Mansur HS, Mansur AA, Curti E, De Almeida MV (2012) Bioconjugation of quantum-dots with chitosan and N,N,N-trimethyl chitosan. Carbohydr Polym 90(1):189–196CrossRef
25.
Gao Z, Shen G, Zhao X, Dong N, Jia P, Wu J, Cui D, Zhang Y, Wang Y (2013) Carbon dots: a safe nanoscale substance for the immunologic system of mice. Nanoscale Res Lett 8(1):276CrossRef
26.
27.
Purcell WT, Ettinger DS (2003) Novel antifolate drugs. Curr Oncol Rep 5(2):114–125CrossRef
28.
Visser K, Van der Heijde D (2009) Risk and management of liver toxicity during methotrexate treatment in rheumatoid and psoriatic arthritis: a systematic review of the literature. Clin Exp Rheumatol 27(6):1017–1025
29.
Grim J, Chládek J, Martínková J (2003) Pharmacokinetics and pharmacodynamics of methotrexate in non-neoplastic diseases. Clin Pharmacokinet 42(2):139–151CrossRef
30.
Abolmaali SS, Tamaddon AM, Dinarvand R (2013) A review of therapeutic challenges and achievements of methotrexate delivery systems for treatment of cancer and rheumatoid arthritis. Cancer Chemother Pharmacol 71(5):1115–1130CrossRef
31.
Chen J, Huang L, Lai H, Lu C, Fang M, Zhang Q, Luo X (2013) Methotrexate-loaded PEGylated chitosan nanoparticles: synthesis, characterization, and in vitro and in vivo antitumoral activity. Mol Pharm 11(7):2213–2223CrossRef
32.
Rahimi M, Shojaei S, Safa KD, Ghasemi Z, Salehi R, Yousefi B, Shafiei-Irannejad V (2017) Biocompatible magnetic tris (2-aminoethyl) amine functionalized nanocrystalline cellulose as a novel nanocarrier for anticancer drug delivery of methotrexate. New J Chem 41(5):2160–2168CrossRef
33.
Yang L, Jiang W, Qiu L, Jiang X, Zuo D, Wang D, Yang L (2015) One pot synthesis of highly luminescent polyethylene glycol anchored carbon dots functionalized with a nuclear localization signal peptide for cell nucleus imaging. Nanoscale 7(14):6104–6113CrossRef
34.
Rezaei SJT, Abandansari HS, Nabid MR, Niknejad H (2014) pH-responsive unimolecular micelles self-assembled from amphiphilic hyperbranched block copolymer for efficient intracellular release of poorly water-soluble anticancer drugs. J Colloid Interface Sci 425:27–35CrossRef
35.
Li W, Li J, Gao J, Li B, Xia Y, Meng Y, Yu Y, Chen H, Dai J, Wang H (2011) The fine-tuning of thermosensitive and degradable polymer micelles for enhancing intracellular uptake and drug release in tumors. Biomaterials 32(15):3832–3844CrossRef
36.
Cao L, Wang X, Meziani MJ, Lu F, Wang H, Luo PG, Lin Y, Harruff BA, Veca LM, Murray D (2007) Carbon dots for multiphoton bioimaging. J Am Chem Soc 129(37):11318–11319CrossRef
37.
Feng T, Ai X, An G, Yang P, Zhao Y (2016) Charge-convertible carbon dots for imaging-guided drug delivery with enhanced in vivo cancer therapeutic efficiency. ACS Nano 10(4):4410–4420CrossRef
38.
Zheng M, Ruan S, Liu S, Sun T, Qu D, Zhao H, Xie Z, Gao H, Jing X, Sun Z (2015) Self-targeting fluorescent carbon dots for diagnosis of brain cancer cells. ACS Nano 9(11):11455–11461CrossRef
39.
Azimi B, Nourpanah P, Rabiee M, Arbab S (2014) Producing gelatin nanoparticles as delivery system for bovine serum albumin. Iran Biomedi J 18(1):34–40
40.
Gao M, Kirstein S, Möhwald H, Rogach AL, Kornowski A, Eychmüller A, Weller H (1998) Strongly photoluminescent CdTe nanocrystals by proper surface modification. J Phys Chem B 102(43):8360–8363CrossRef
41.
Zhang H, Zhou Z, Yang B, Gao M (2003) The influence of carboxyl groups on the photoluminescence of mercaptocarboxylic acid-stabilized CdTe nanoparticles. J Phys Chem B 107(1):8–13CrossRef
42.
Rahman M, Dey K, Parvin F, Sharmin N, Khan RA, Sarker B, Nahar S, Ghoshal S, Khan M, Billah MM (2011) Preparation and characterization of gelatin-based PVA film: effect of gamma irradiation. Int J Polym Mater 60(13):1056–1069CrossRef
43.
Peng H, Xiong H, Li J, Xie M, Liu Y, Bai C, Chen L (2010) Vanillin cross-linked chitosan microspheres for controlled release of resveratrol. Food Chem 121(1):23–28CrossRef
44.
Karthikeyan S, Prasad NR, Ganamani A, Balamurugan E (2013) Anticancer activity of resveratrol-loaded gelatin nanoparticles on NCI-H460 non-small cell lung cancer cells. Biomed Prev Nutr 3(1):64–73CrossRef
45.
Wang Y, Zhao Q, Han N, Bai L, Li J, Liu J, Che E, Hu L, Zhang Q, Jiang T (2015) Mesoporous silica nanoparticles in drug delivery and biomedical applications. Nanomed Nanotechnol Biol Med 11(2):313–327CrossRef
46.
Bajpai A, Choubey J (2005) Release study of sulphamethoxazole controlled by swelling of gelatin nanoparticles and drug-biopolymer interaction. J Macromol Sci Part A Pure Appl Chem 42(3):253–275CrossRef
47.
Kimelberg HK, Tracy TF, Biddlecome SM, Bourke RS (1976) The effect of entrapment in liposomes on the in vivo distribution of [3H] methotrexate in a primate. Cancer Res 36(8):2949–2957
48.
Guo M, Yan Y, Liu X, Yan H, Liu K, Zhang H, Cao Y (2010) Multilayer nanoparticles with a magnetite core and a polycation inner shell as pH-responsive carriers for drug delivery. Nanoscale 2(3):434–441CrossRef
49.
Meloun M, Ferenčíková Z, Vrána A (2010) The thermodynamic dissociation constants of methotrexate by the nonlinear regression and factor analysis of multiwavelength spectrophotometric pH-titration data. Open Chem 8(3):494–507CrossRef
50.
Ghorbani M, Hamishehkar H, Arsalani N, Entezami AA (2016) A novel dual-responsive core-crosslinked magnetic-gold nanogel for triggered drug release. Mater Sci Eng, C 68:436–444CrossRef
51.
Ghorbani M, Hamishehkar H (2017) Redox and pH-responsive gold nanoparticles as a new platform for simultaneous triple anti-cancer drugs targeting. Int J Pharm 520(1):126–138CrossRef
Metadaten
Titel
Development of biocompatible fluorescent gelatin nanocarriers for cell imaging and anticancer drug targeting
verfasst von
Parinaz Nezhad-Mokhtari
Nasser Arsalani
Marjan Ghorbani
Hamed Hamishehkar
Publikationsdatum
04.05.2018
Verlag
Springer US
Erschienen in
Journal of Materials Science / Ausgabe 15/2018
Print ISSN: 0022-2461
Elektronische ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-018-2371-8

Weitere Artikel der Ausgabe 15/2018

Journal of Materials Science 15/2018 Zur Ausgabe

Energy materials

Enhancement of electrochemical performance of lithium-ion battery by single-ion conducting polymer addition in ceramic-coated separator

Chemical routes to materials

Effect of coordinated water of hexahydrate on nickel platings from choline–urea ionic liquid

Composites

Improved properties of PLA biocomposites toughened with bamboo cellulose nanowhiskers through silane modification

Energy materials

Mangosteen peel-derived porous carbon: synthesis and its application in the sulfur cathode for lithium sulfur battery

Ceramics

Microcrystals of antimony compounds in lead–potassium and lead glass and their effect on glass corrosion: a study of historical glass beads using electron microscopy

Electronic materials

Controlled fabrication of TiO2/C3N4 core–shell nanowire arrays: a visible-light-responsive and environmental-friendly electrode for photoelectrocatalytic degradation of bisphenol A

Premium Partner

    Marktübersichten

    Die im Laufe eines Jahres in der „adhäsion“ veröffentlichten Marktübersichten helfen Anwendern verschiedenster Branchen, sich einen gezielten Überblick über Lieferantenangebote zu verschaffen. 

    Zur Marktübersicht
    Bildnachweise