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Biomass Conversion and Biorefinery
Erschienen in: Biomass Conversion and Biorefinery 7/2022

06.06.2020 | Original Article

Production of cellulolytic enzymes by Myceliophthora thermophila and their applicability in saccharification of rice straw

verfasst von: Anu, Anil Kumar, Davender Singh, Vinod Kumar, Bijender Singh

Erschienen in: Biomass Conversion and Biorefinery | Ausgabe 7/2022

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Abstract

Optimization of cellulase production by Myceliophthora thermophila BJTLRMDU3 was studied in solid state fermentation. Sequential use of Plackett-Burman and response surface methodology (RSM) resulted in the production of 98.81, 243.19, and 316.48 U/g dry moldy residue (DMR) of FPase, CMCase, and β-glucosidase, respectively. Statistical optimization has resulted in more than 4.0-fold increase in the production of cellulases. Optimization of saccharification of untreated and pretreated rice straw was carried out by cellulolytic enzymes of thermophilic mold M. thermophila. The liberation of reducing sugars was higher using fungal cellulases in ammonia-pretreated rice straw as compared with untreated biomass. Maximum liberation of reducing sugars was attained at 60 °C (224.24 mg/g substrate) and pH 5.0 (246.33 mg/g substrate) after an incubation time of 24 h (345.61 mg/g substrate) using enzyme dose of 20 U/g in ammonia-pretreated rice straw. Supplementation of xylanase further enhanced the saccharification (488.78 mg/g substrate) of pretreated rice straw. Analysis using high-performance liquid chromatography (HPLC) indicated the presence of various monomeric and oligomeric sugars in the enzymatic hydrolysate of pretreated rice straw. Both, Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) revealed the structural changes in rice straw after ammonia pretreatment.
Vorheriger Artikel Effects of pyrolysis temperature on production and physicochemical characterization of biochar derived from coconut fiber biomass through slow pyrolysis process
Nächster Artikel Pyrolysis of olive cake with catalytic upgrading of volatile products

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Metadaten
Titel
Production of cellulolytic enzymes by Myceliophthora thermophila and their applicability in saccharification of rice straw
verfasst von
Anu
Anil Kumar
Davender Singh
Vinod Kumar
Bijender Singh
Publikationsdatum
06.06.2020
Verlag
Springer Berlin Heidelberg
Erschienen in
Biomass Conversion and Biorefinery / Ausgabe 7/2022
Print ISSN: 2190-6815
Elektronische ISSN: 2190-6823
DOI
https://doi.org/10.1007/s13399-020-00783-1

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